The botanical name of Upland 
(American) cotton is Gossypium hersutum and it belong to family Malvaceae. 
Amerian cotton is tetraploid specie and chromosome number is 2n=52The word BT 
mean Bacillus thurngenises. This is an Agro bacterium cause crown gall on cotton 
plant. BT gene extracted from Agro bacterium and transfer in cotton plant 
through genetic engineering. This BT gene produces resistance against boll worm 
pests.
Cotton is an important cash crop for Pakistan known as “white gold”. It accounts 
for 8.2 percent of the value added in agriculture and about 3.2 percent to GDP; 
around two thirds of the country’s export earnings from the cotton made-up and 
textiles which adds over $2.5 billion to the national economy; while hundreds of 
ginning factories and textile mills in the country heavily depends upon cotton. 
Life of several of farmers is dependent on this crop, in addition to millions of 
people employed in the entire cotton value chain, from weaving to textile and 
garment exports. Pakistan in the world fourth-largest cotton producer, third 
largest exporter of raw cotton and a leading exporter of yarn in the world our 
yield per acres ranks 13th in the world; as a result Pakistan annually imports 
app. 1.5-2.00 million bales of cotton to meet growing demand from local textile 
mills; therefore it has become vital for Pakistan to increase its yield per 
acre. 
Agricultural biotechnology is playing an important role to gain better crops and 
food for bio-safety. So, many farmers in the world are focusing genetic 
engineered varieties (GE) to improve cotton yield. American multinational 
Monsanto Company develops genetically engineered first BT cotton variety, 
Firstly BT cotton seed named as BT-121 smuggled from neighbouring country-India 
and grown in Upper Sindh.
If we seen at the Pakistan problems, there are two major types of pests which 
are damaging cotton crops – sucking and chewing; sucking pests controlled by 
strong pesticides but major challenge to control chewing pests i.e. known as 
Bollworms – American, Army, Pink and Spotted bollworm are major causes to 
decrease cotton crop yield and lint quality. Another recent disease cotton leaf 
curl virus (CLCV) spread in Punjab and Sindh pushed our institutes.
Pakistan also realizes the significance of BT Cotton, and the top political 
leadership including the Prime Minister Shaukat Aziz himself has said that 
government would allow to farmers to BT cotton grown openly, which confirms 
government’s policy of being open to genetically modified crops in the near 
future. Other ministers have also spoken in favor of adopting the BT Cotton.
But in order to benefit from its true potential, government and all key 
stakeholders including farmers, seed companies, R & D institutes, seed dealers 
and traders are involved in commercial activity for Agricultural development. If 
we choose to ignore regulatory frameworks that govern the chain of activity, and 
international agreements on Biosafety that Pakistan is signatory to, to make 
quick bucks or in an attempt to provide a speedy access of technology to the 
farmers, we shall end up losing the benefits from the technology in the long run 
by undermining it. We have to ensure effective, stringent, and transparent 
enforcement of Biosafety Rules 2005, Seed Act 1976, and Punjab Cotton Control 
Ordinance, to encourage the introduction of technology through legal means with 
its complete package of benefits.
These rules setup legal requirements for import, export, transport, and handling 
of biological agents, genetic engineering organisms or vectors, seeds, crops and 
foods, besides setting conditions for the researchers; seeds developers and 
companies. Usually it takes two to three years to do proper assessment for any 
Biotech crops before its commercialization.
Recently in Pakistan like Pakistan Atomic Energy Commission (PAEC), National 
Institute for Biotechnology and Genetic Engineering (NIBGE), Nuclear Institute 
of Agriculture and Biology (NIAB), Agricultural Biotechnology research institute 
Faisalabad,Cotton research institute Faisalabad,Cotton research institute 
Multan,Centre of Agricultural Biotechnology and Biochemistry (CABB) UAF and 
National center of Excellence in Molecular Biology (NCEMB) at Punjab University 
Lahore to cope with such problems; significant amount of financial resources and 
manpower have been committed by the Government of Pakistan for developing 
genetically modified (GM) local cotton varieties. 
Thus different Pakistani Agricultural research institute released different BT 
varieties for Biosafety i.e. “IR-FH-901”, “IR-NIBGE-2”, “IR-CIM-448” and 
“IR-CIM-443”; and recently released NIAB-886 and NIAB-777.These BT cotton 
varieties have been tightly screened and evaluated by PAEC on the bases of their 
capacity to follow Bio-safety rules. Cotton research institute 
Faisalabad.released FH-113 and FH-114 BT varieties. These varieties screened and 
evaluated by Pakistan Agricultural research council.
Although Pakistani BT cotton varieties good staple length (22mm-30mm) and 
fitness, germination of these BT cotton seed varieties vary from 55 % to 80 %, 
but mixing or impurities were less than 2 %.
BT cotton varieties significantly more yielded per acre as compare to non Bt 
cotton varieties i.e. up to 40 mound (1 Mound = 40 Kg) of BT cotton per acres 
but 17-20 mound per acre to traditional cotton varieties. This translates into 
more than 35 percent increase in yield. It is noted that in BT cotton crops 
average number of cotton Bolls per plant are 125 while average Boll weight is 
appro. 1.95 grams including seeds and number of plants per acre are as 
recommended by the department of agriculture. In Pakistan average cotton grower 
has 10 acres of land; increase in such small income per acre would improve his 
quality of life. Recently, it is expected that more than 80% cotton area covered 
by BT varieties in Pakistan Punjab Cotton belt farmers ( Bahawalpur, Multan, 
Muzaffer Garh and Karor Pakka;) observed and evaluated independently its 
resistance and susceptibility to different biotic stress, abiotic stress and 
yield compared it with non Bt cotton varieties grown in the same locations. A 
large number of farmers have visited these fields, and become aware of the 
benefits of the locally developed BT cotton. 
Although, the overall attack of “Army boll worm” “American bollworm” and other 
bollworms remained low as compared to previous years but attack of sucking pests 
like Jassid, Whitefly, Thrips and other Aphid were high in both BT and non BT 
cotton crops. No serious incidence of cotton leaf curl virus disease was 
reported in BT cotton varieties. Heat stress in cotton crop was also recorded in 
different region, Army, Pink and Spotted bollworm were active from July to 
October but severe attack during September and Army boll worm remained in the 
field after spray for limited time period. 
It is also important that newly hatched Helicoverpa Armigera when eat leaves of 
the BT cotton plants, they died after few days; no larvae reached to pupal stage 
while in non BT it has developed into pupa. Significant number of spray has 
decline in BT cotton varieties; 4 – 5 applications as compare to non BT cotton 
crop 6 – 9; most of chemicals application used to control sucking pests; however 
in chewing; pests spray was required to control Army boll worm. Although BT 
cotton also play significant control the target bollworms but partial foliar 
insecticide sprays are required to control other bollworms from causing 
excessive damage in BT fields. 
Right dose of BT toxin “Cry-protein” in very crucial in each BT seed; if we grow 
the same BT seed repeatly on same location; the right dose of BT toxin will be 
reduced and cotton plant susceptible to pest. So it will harmful for our 
agriculture, may be hazard for health and environment.
Besides the cotton lint; more than 70% of edible oil is extracted from 
cottonseed and in addition to that more than 2 million tons of oil seedcake is 
also produced as a feed for livestock and poultry.
WTO passed the rule that all the Biotech. Applications in crops and food being 
decided on scientific rather than political grounds for human and animal health 
Being health safety studies prove that, BT cotton oil, leaves and feed has no 
health hazards and side effects on human, animals and it safe for environment. 
These studies created a positive consciousness and confidence between the 
international and Pakistani scientists and people about BT cotton.
Today, all major cotton producing countries are benefiting from the cultivation 
of BT Cotton. In the last season Up to 70 percent of BT cotton in USA, 76 
percent in China and 80 percent in Australia ,more than 80 percent in Pakistan 
and India grown with “single” or “double” BT gene technology. It is expected 
that within two years more than half of the world’s cotton may be grown from 
genetically modified crops. 
BT genotypes are highly dependent on agro climatic conditions, plant genetics 
and crop management. In some areas, certain BT varieties may be show non 
significant result, for example under high night temperatures and under hot dry 
conditions, bolls may drop off the plants. Therefore use only those approved BT 
cotton varieties; which are screened under your local agro-climatic conditions.
And the scientists have no claimed that BT cotton varieties increase yield due 
to presence of genetically modified organism; however, the increase in yield can 
be gain by crop protection from boll worm pests.